Tabriz University of Medical Sciences ImmunoAnalysis 2783-2589 4 1 2024 01 20 Matrix Metallopeptidase 9 Expression in Tumoral and Marginal Tissues of Cholesteatoma 8 8 10.34172/ia.4084 EN Yalda Jabbari Moghaddam https://orcid.org/0000-0003-3217-8391 Masoud Naderpour Germi https://orcid.org/0000-0001-6552-2166 Shabnam Noei Alamdary https://orcid.org/0000-0003-4622-7852 Milad Emami Dariush Shanehbandi Saiedeh Razi Soofiyani Journal Article 10.34172/ia.4084 2024 01 24 2024 06 05 Background: Cholesteatoma, a serious, expanding otolaryngologic condition, is challenging to otolaryngologists worldwide. The purpose of the present study was to evaluate the expression of MMP-9 gene in cholesteatoma patients. Methods: In this study, 40 samples of tumor tissue and 40 samples as control of tumor margins of patients with cholesteatoma were examined. Quantitative real-time polymerase chain reaction (PCR) was used for detection of the MMP-9 gene expression levels in cholesteatoma and control group. expression changes were calculated by ΔCT formula and data were analyzed by GraphPad Prism 8. Results: MMP-9 expression level in case group was significantly higher than the control group (P=0.0011). ROC analysis for the MMP-9 gene expression level that discriminates Cholesteatoma tumoral tissue from healthy tissues revealed a sensitivity of 67.5 % and a specificity of 84.62% with an area under the curve (AUC) value of 0.75 (P<0.001). Conclusion: The results indicated that MMP-9 is overexpressed in cholesteatoma tumoral tissues group compared to the healthy tissues. Based on AUC value of RUC curve analysis of MMP-9 expression level could be helpful in diagnosis of tumoral tissue form healthy tissue.